Rapid identification of carbapenemase-type bla

Autor:	Shizuo, Kayama, Raita, Yano, Katsutoshi, Yamasaki, Chiemi, Fukuda, Keiko, Nishimura, Hitoshi, Miyamoto, Hiroki, Ohge, Motoyuki, Sugai
Rok vydání:	2018
Předmět:	Bacteriological Techniques Time Factors Japan Gram-Negative Bacteria Humans Gram-Negative Bacterial Infections Multiplex Polymerase Chain Reaction beta-Lactamases
Zdroj:	Journal of microbiological methods. 148
ISSN:	1872-8359
Popis:	Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::47da2927087f5b4c6bee01fb339f266d https://pubmed.ncbi.nlm.nih.gov/29605523 Zobrazit plný text záznamu Full Text from ScienceDirect