| Autor: |
P, Darlington, H, Haugom-Olsen, K, von Sivers, J, Wahlström, M, Runold, V, Svjatoha, A, Porwit, A, Eklund, J, Grunewald |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Journal of internal medicine. 272(5) |
| ISSN: |
1365-2796 |
| Popis: |
An increased percentage of CD4+ T cells is usually observed in bronchoalveolar lavage fluid (BALF) from patients with sarcoidosis. In HLA-DRB1*03-positive patients, such T cells express the T-cell receptor (TCR) AV2S3+ gene segment. It is not known whether cells found in BALF reflect those in enlarged regional lymph nodes (LNs). Therefore, the aim of this study was to compare T-cell phenotypes in BALF, blood and mediastinal LNs.Fifteen patients underwent clinical investigation including bronchoscopy with bronchoalveolar lavage. Blood samples were drawn, and endoscopic ultrasound-guided fine-needle aspiration of enlarged mediastinal LNs was performed via the oesophagus. T cells from all three compartments were analysed by flow cytometry for markers of activity, differentiation and T regulatory function.The CD4/CD8 ratio was significantly higher in BALF compared with regional LNs and was also significantly higher in LNs than in blood. The CD4+ T cells were recently activated and more differentiated in BALF than in blood and LNs. There was an accumulation of T regulatory cells (FOXP3+) in LNs and a correlation between high levels of FOXP3+ cells in BALF and in LNs. In HLA-DRB1*03-positive patients, TCR AV2S3+ CD4+ T cells were predominantly localized within BALF.The CD4+ T-cell phenotype in BALF indicates an active ongoing specific immune response primarily localized to the alveolar space. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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