| Autor: |
Chuan, Li, Wei, Jiang, Zhuo-Gang, Liu, Pei-Qi, Liang, Rong, Hu |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 27(6) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the effect of reactive oxygen species (ROS) on GDC-0152-induced apoptosis and autophagy of acute promyelocytic leukemia cell line NB4.Different concentrations of GDC-0152 combined with Z-VAD-FMK was applied to NB4 cells. Cell proliferation was detected by CCK8 method. Apoptosis rate, autophagy and ROS level were detected by flow cytometry. The autophagy was observed by Cyto-ID staining fluorescence microscopy, and flow cytometry were used to detect the fluorescence expression. The expression of autophagy-related protein LC3B was detected by Western blot.GDC-0152 increased proliferation inhibition rate and apoptosis rate in NB4 cells (P＜0.05); GDC-0152 induced increase of ROS level of NB4 cells; GDC-0152 increased autophagy of NB4 cells that was found by Cyto-ID staining fluorescence microscopy and flow cytometry (P＜0.05). Western blot showed that GDC-0152 increased LC3B expression in NB4 cells and promoted the conversion of LC3BI to LC3BII; as compared with GDC-0152 (100 ng/ml), GDC-0152 (100 ng/ml) combined with ROS inhibitor YCG063 (10 μmol/L) decreased apoptosis and autophagy (P＜0.05).GDC-0152 inhibits cell proliferation by inducing apoptosis and autophagy of NB4 cells. ROS can promote GDC-0152-induced apoptosis and autophagy of NB4 cells.活性氧在GDC-0152诱导NB4细胞凋亡和自噬中的作用.探讨活性氧（Reactive oxygen species, ROS）在GDC-0152诱导急性早幼粒细胞白血病细胞系NB4细胞凋亡和自噬过程中的作用.不同浓度GDC-0152联合Z-VAD-FMK作用于NB4细胞，采用CCK8法检测细胞增殖活力；流式细胞术检测细胞凋亡率、细胞自噬及ROS水平；Cyto-ID染色荧光显微镜观察细胞自噬及流式细胞术检测荧光表达；Western blot 检测自噬相关蛋白LC3B的表达.GDC-0152处理NB4细胞后细胞增殖抑制率和细胞凋亡率增加（P＜0.05）；GDC-0152可诱导NB4细胞ROS水平升高；Cyto-ID 染色后应用荧光显微镜观察和流式细胞术检测发现， GDC-0152 可以增加 NB4 细胞自噬（P＜0.05）；Western blot结果显示，GDC-0152可增加NB4细胞LC3B表达，且促进LC3BⅠ向LC3BⅡ转化;与GDC-0152（100 ng/ml）相比，GDC-0152（100 ng/ml）联合ROS抑制剂YCG063（10 μmol/L）后细胞凋亡率降低且自噬减少（P＜0.05）.GDC-0152通过诱导NB4细胞凋亡及自噬抑制细胞增殖。ROS能促进GDC-0152诱导的NB4细胞凋亡和自噬. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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