Selective silencing of full-length CD80 but not IgV-CD80 leads to impaired clonal deletion of self-reactive T cells and altered regulation of immune responses

Autor:	L, Bugeon, R E, Hargreaves, T, Crompton, S, Outram, A, Rahemtulla, A C, Porter, M J, Dallman
Rok vydání:	2001
Předmět:	Mice Inbred C3H T-Lymphocytes Immunoglobulin Variable Region Clonal Deletion CHO Cells Lymphocyte Activation Mice Inbred C57BL Mice Mice Inbred DBA Cricetinae Gene Targeting Mutation B7-1 Antigen Animals RNA Messenger
Zdroj:	European journal of immunology. 31(1)
ISSN:	0014-2980
Popis:	Co-stimulation provided by the B7 family of proteins underpins the development of protective immunity. There are three identified members of this family: CD80, its splice variant IgV-CD80 and CD86. It has hitherto been difficult to analyze the expression and function of IgV-CD80 since there are no appropriate reagents capable of distinguishing it from CD80. We have generated mice, by gene targeting, the lack CD80 whilst maintaining expression of IgV-CD80. Mutant animals did not delete T cells bearing mammary tumor virus-reactive TCR as efficiently as wild-type animals. We also demonstrate the importance of IgV-CD80 in the responses of recently activated cells and reveal a role for CD80 in sustaining T cell responses. CD86, whilst critical to primary T cell activation, made only a minor contribution to re-activation of normal cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4727e68c8dd0583b75e82d1b3759b128 https://pubmed.ncbi.nlm.nih.gov/11169445 Zobrazit plný text záznamu Plný text