Autor: |
V, Tollemar, N, Tudzarovski, E, Boberg, A, Törnqvist Andrén, A, Al-Adili, K, Le Blanc, K, Garming Legert, M, Bottai, G, Warfvinge, R V, Sugars |
Rok vydání: |
2018 |
Předmět: |
|
Zdroj: |
Cytometry. Part A : the journal of the International Society for Analytical Cytology. 93(10) |
ISSN: |
1552-4930 |
Popis: |
Visual grading of chromogenically stained immunohistochemical (IHC) samples is subjective, time consuming, and predisposed to considerable inter- and intra-observer variations. The open-source digital analysis software, CellProfiler has been extensively used for fluorescently stained cells/tissues; however, chromogenic IHC staining is routinely used in both pathological and research diagnostics. The current investigation aimed to compare CellProfiler quantitative chromogenic IHC analyses against the gold standard manual counting. Oral mucosal biopsies from patients with chronic graft-versus-host disease were stained for CD4. Digitized images were manually counted and subjected to image analysis in CellProfiler. Inter-observer and inter-platform agreements were assessed by scatterplots with linear regression and Bland-Altman plots. Validation comparisons between the manual counters demonstrated strong intra-observer concordance (r |
Databáze: |
OpenAIRE |
Externí odkaz: |
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