| Autor: |
M, Vrkljan, T M, Foster, M E, Powers, J, Henkin, W R, Porter, H, Staack, J F, Carpenter, M C, Manning |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Pharmaceutical research. 11(7) |
| ISSN: |
0724-8741 |
| Popis: |
Turbidimetric or light scattering assays can be used to determine the extent of aggregation in protein formulations. Using low molecular weight urokinase (LMW-UK) as a model protein, the effect of polymeric additives on heat-induced aggregation was evaluated. Previous work has shown that under 60 degrees C heat treatment, LMW-UK initially denatures and the unfolded protein associates to form soluble aggregates. Eventually, these aggregates associate to form a precipitate. The effects of polymers on the initial aggregation phase was examined. Hydroxyethyl (heta) starch, polyethylene glycol 4000, and gelatin were found to be effective, concentration-dependent inhibitors of aggregation, whereas polyvinylpyrrolidone (PVP) and polyethylene glycol 300 were ineffective. Overall, the effect of polymeric additives on the stability of thermally-stressed LMW-UK can be accounted for by preferential exclusion of the solute from the surface of the protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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