[Effect of hydrogen ion concentration in the medium on the state of chromatin in lymphoid cells]

Autor:	B I, Sukhorukov, P M, Shvartsburd
Rok vydání:	1985
Předmět:	Luminescence Spectrophotometry Infrared Protein Conformation Cell Membrane Humans DNA Lymphocytes Hydrogen-Ion Concentration Cells Cultured Chromatin Culture Media
Zdroj:	Biofizika. 30(4)
ISSN:	0006-3029
Popis:	Effect of medium acidity (pH 7.2 divided by 6.6) on the state of lymphoid tissue cells was studied by microspectrofluorimetry, electrophoresis and IR-spectroscopy. Two stages of the cell structural-functional changes were found at H2CO3 acidification of the medium. The first one is a reversible transition of chromatin from the state of dense sphere to the margination state when chromatin is located along the membrane. The second one is an irreversible destruction of chromatin and the cell death. The margination stage is related to the formation of non-specific DNA-membrane contacts, while the chromatin destruction stage to the "proton breakdown" of the macromolecules and their supramolecular complexes. A relationship between the degree of margination reversibility and the value of the medium acidification and the time of cell incubation in it is shown. Different functional significance of chromatin marginated state is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::46d36d5fef76bf9fed9e96c0b911645a https://pubmed.ncbi.nlm.nih.gov/4052466 Zobrazit plný text záznamu