PCR amplification of streptococcal DNA using crude cell lysates

Autor:	W L, Hynes, J J, Ferretti, M S, Gilmore, R A, Segarra
Rok vydání:	1992
Předmět:	DNA Bacterial Base Sequence Streptococcus pyogenes Detergents Endopeptidases Molecular Sequence Data Enterococcus faecalis Cell Fractionation Polymerase Chain Reaction
Zdroj:	FEMS microbiology letters. 73(1-2)
ISSN:	0378-1097
Popis:	Gram-positive organisms such as streptococci and enterococci are often difficult to lyse. Obtaining DNA for procedures such as PCR amplification usually requires a large scale isolation for each strain under investigation. We describe a simple procedure for small volumes of whole cells, involving pretreatment with detergent and proteinase that allows for efficient release of DNA for PCR amplification. This procedure is fast, reproducible, can be used with a large number of samples, and has been successfully applied to a variety of streptococcal and enterococcal strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::46955bd54170bcc6e1cc7ce81e891100 https://pubmed.ncbi.nlm.nih.gov/1521762 Zobrazit plný text záznamu Plný text