Drought Stress Tolerance and Photosynthetic Activity of Alloplasmic Lines
| Autor: | Nina V, Terletskaya, Andrey B, Shcherban, Michail A, Nesterov, Roman N, Perfil'ev, Elena A, Salina, Nazira A, Altayeva, Irina V, Blavachinskaya |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetic Markers
Genotype DREB drought tolerance Genes Plant DNA Mitochondrial Article Electron Transport Stress Physiological Photosynthesis Alleles Crosses Genetic Triticum Plant Proteins Cell Nucleus fungi food and beverages Photosystem II Protein Complex Water rps19-p genes SSR Droughts orf256 Plant Leaves Plant Breeding Seedlings alloplasmic wheat lines Microsatellite Repeats Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | Tetraploid species T. dicoccum Shuebl is a potential source of drought tolerance for cultivated wheat, including common wheat. This paper describes the genotyping of nine stable allolines isolated in the offspring from crossing of T. dicoccum x T. aestivum L. using 21 microsatellite (simple sequence repeats—SSR) markers and two cytoplasmic mitochondrial markers to orf256, rps19-p genes; evaluation of drought tolerance of allolines at different stages of ontogenesis (growth parameters, relative water content, quantum efficiency of Photosystem II, electron transport rate, energy dissipated in Photosystem II); and the study of drought tolerance regulator gene Dreb-1 with allele-specific PCR (AS-MARKER) and partial sequence analysis. Most allolines differ in genomic composition and T. dicoccum introgressions. Four allolines—D-b-05, D-d-05, D-d-05b, and D-41-05—revealed signs of drought tolerance of varying degrees. The more drought tolerant D-41-05 line was also characterized by Dreb-B1 allele introgression from T. dicoccum. A number of non-specific patterns and significant differences in allolines in regulation of physiological parameters in drought conditions is identified. Changes in photosynthetic activity in stress-drought are shown to reflect the level of drought tolerance of the forms studied. The contribution of different combinations of nuclear/cytoplasmic genome and alleles of Dreb-1 gene in allolines to the formation of stress tolerance and photosynthetic activity is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|