Imaging of HER2 with [
| Autor: | Adriana V F, Massicano, Supum, Lee, Bryant K, Crenshaw, Tolulope A, Aweda, Retta, El Sayed, Ian, Super, Ron, Bose, Bernadette V, Marquez-Nostra, Suzanne E, Lapi |
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| Rok vydání: | 2019 |
| Předmět: |
Radioisotopes
Receptor ErbB-2 Mice Nude Breast Neoplasms X-Ray Microtomography Original Articles Deferoxamine Trastuzumab Ado-Trastuzumab Emtansine Antibodies Monoclonal Humanized Xenograft Model Antitumor Assays Tumor Burden Mice Treatment Outcome Isothiocyanates Cell Line Tumor Positron Emission Tomography Computed Tomography Animals Humans Female Maytansine Zirconium Radiopharmaceuticals skin and connective tissue diseases |
| Zdroj: | Cancer Biother Radiopharm |
| ISSN: | 1557-8852 |
| Popis: | Background: The success of human epidermal growth factor receptor 2 (HER2)-targeted therapy depends on accurate characterization of HER2 expression, but current methods available have several limitations. This study aims to investigate the feasibility of [(89)Zr]pertuzumab imaging to monitor early response to Ado-trastuzumab emtansine (T-DM1) therapy in mice bearing xenografts of HER2-positive breast cancer (BCa). Materials and Methods: Pertuzumab was conjugated to DFO-Bz-NCS and labeled with (89)Zr. Mice bearing BT-474 tumors were imaged with [(89)Zr]pertuzumab and [(18)F]FDG before and after T-DM1 therapy. Results: Pertuzumab was successfully labeled with (89)Zr with a specific activity of 0.740 MBq/μg. Overall [(18)F]FDG images showed poor delineation of tumors. Using [(18)F]FDG-PET to measure tumor volume, the volume remained unchanged from 107.6 ± 20.7 mm(3) before treatment to 89.87 ± 66.55 mm(3) after treatment. In contrast, [(89)Zr]pertuzumab images showed good delineation of HER2-positive tumors, allowing accurate detection of changes in tumor volume (from 243.80 ± 40.91 mm(3) before treatment to 78.4 ± 40.43 mm(3) after treatment). Conclusion: [(89)Zr]pertuzumab may be an imaging probe for monitoring the response of HER2-positive BCa patients to T-DM1 therapy. |
| Databáze: | OpenAIRE |
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