Characterization of closely related delta-TIP genes encoding aquaporins which are differentially expressed in sunflower roots upon water deprivation through exposure to air
| Autor: | X, Sarda, D, Tousch, K, Ferrare, F, Cellier, C, Alcon, J M, Dupuis, F, Casse, T, Lamaze |
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| Rok vydání: | 1999 |
| Předmět: |
Osmosis
Cell Membrane Permeability DNA Complementary Base Sequence Sequence Homology Amino Acid Arabidopsis Proteins Xenopus Molecular Sequence Data Porins Water Biological Transport Aquaporins Blotting Northern Genes Plant Plant Roots Polymerase Chain Reaction Gene Expression Regulation Plant Sequence Homology Nucleic Acid Oocytes Animals Helianthus Tissue Distribution Amino Acid Sequence Gene Library Plant Proteins |
| Zdroj: | Plant molecular biology. 40(1) |
| ISSN: | 0167-4412 |
| Popis: | We isolated five sunflower (Helianthus annuus) cDNAs belonging to the TIP (tonoplast intrinsic protein) family. SunRb7 and Sun gammaTIP (partial sequence) are homologous to tobacco TobRb7 and Arabidopsis gamma-TIP, respectively. SunTIP7, 18 and 20 (SunTIPs) are closely related and homologous to Arabidopsis delta-TIP (SunTIP7 and 20 have already been presented in Sarda et al., Plant J. 12 (1997) 1103-1111). As was previously shown for SunTIP7 and 20, expression of SunTIP18 and SunRb7 in Xenopus oocytes caused an increase in osmotic water permeability demonstrating that they are aquaporins. In roots, in situ hybridization revealed that SunTIP7 and 18 mRNAs accumulate in phloem tissues. The expression of TIP-like genes was studied in roots during 24 h water deprivation through exposure to air. During the course of the treatment, each SunTIP gene displayed an individual response: SunTIP7 transcript abundance increased, SunTIP18 decreased whereas that of SunTIP20 was transitorily enhanced. By contrast, SunRb7 and Sun gammaTIP mRNA levels did not fluctuate. Due to the changes in their transcript levels, it is proposed that SUNTIP aquaporins encoded by delta-TIP-like genes play a role in the sunflower response to drought. |
| Databáze: | OpenAIRE |
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