| Autor: |
S N, Vogel, R M, Friedman, M M, Hogan |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Current protocols in immunology. |
| ISSN: |
1934-368X |
| Popis: |
The Basic Protocol in this unit describes an assay for murine IFN-induced antiviral activity and employs vesicular stomatitis virus (VSV) and IFN-sensitive fibroblasts. Support Protocol describes the preparation of VSV cultures and the calculation of multiplicity of infection (MOI; i.e., concentration of viral particles required to infect cells). Support Protocol describes the antibody neutralization assay, which can be used to identify the bioactive species of IFN in a sample, or to test the potency of an antibody preparation against a particular species of IFN. Alternate Protocol 1 covers measurement of human IFN-induced antiviral activity; while the steps are quite similar to Basic Protocol, different viral and cell cultures are described. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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