| Autor: |
K, Kamisango, H, Fujii, H, Okumura, I, Saiki, Y, Araki, Y, Yamamura, I, Azuma |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Journal of biochemistry. 93(5) |
| ISSN: |
0021-924X |
| Popis: |
An immunologically active teichoic acid component was isolated from the cell wall of Listeria monocytogenes strain EGD. The teichoic acid component, accounting for about 20% of the weight of cell wall, contained N-acetylglucosamine, rhamnose, ribitol, and phosphorus in a molar ratio of 0.95 : 1.0 : 0.97 : 0.98. The molecular weight of the teichoic acid chain was about 120,000 as analyzed by gel filtration. The probable structure was deduced from the results of methylation analysis, Smith degradation, and proton magnetic resonance spectrometry of the teichoic acid, together with the characterization of fragments obtained by treatment with hydrofluoric acid, as follows: (formula; see text) Inhibition testing with monosaccharide and fragments obtained from HF treatment of Listeria teichoic acid in the quantitative precipitin reaction suggested that the rhamnose residue is a major antigenic determinant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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