| Autor: |
Z A, Darieva, V A, Pospelov, T V, Pospelova |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Tsitologiia. 41(7) |
| ISSN: |
0041-3771 |
| Popis: |
DNA binding activity and intracellular distribution of transcription factor NF-kappa B in primary embryo fibroblast (REF) cells transformed by the complementing oncogenes E1A plus cHa-ras have been studied. By means of a gel retardation assay with a regulatory element kappa B, we showed that DNA binding activity of NF-kappa B increased upon transformation. In contrast to normal REF cells, the elevated activity of NF-kappa B in the transformants cannot be regulated by growth factors of serum and phorbol ester. Moreover, an immunofluorescence study showed that the main component of NF-kappa B factor--p65/Re1 protein--was constitutively localized in the nucleus of E1A + cHa-ras transformants independently on the growth conditions: serum starvation or serum stimulation. Mechanisms of constitutive activation of transcription factor NF-kappa B upon transformation of REF cells by E1A + cHa-ras oncogenes have been discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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