C. elegans nuclear receptor NHR-6 functionally interacts with the jun-1 transcription factor during spermatheca development

Autor:	Chris R, Gissendanner, Derrick, Cardin, Chris J, Dubose, Moustafa, El Sayed, Jeremy S, Harmson, Brandon, Praslicka, Brian G, Rowan
Rok vydání:	2013
Předmět:	animal structures Organogenesis Receptors Cytoplasmic and Nuclear Article Animals Genetically Modified Two-Hybrid System Techniques Animals Female RNA Interference Caenorhabditis elegans Caenorhabditis elegans Proteins Gonads Genes Helminth Transcription Factors
Zdroj:	Genesis (New York, N.Y. : 2000). 52(1)
ISSN:	1526-968X
Popis:	The NR4A nuclear receptor NHR-6 is an essential regulator of spermatheca organogenesis in C. elegans. In this study, we perform a focused, RNAi-based screen to identify modifiers of partial nhr-6 loss of function. Ninety-eight genes that encode signaling proteins expressed in the spermatheca were screened for enhancement of the nhr-6 RNAi phenotype. We identify the C. elegans gene jun-1, which encodes the homolog of the Jun transcription factor, as a strong enhancer of nhr-6 partial loss of function. We show that nhr-6 and jun-1 function together to regulate development of the spermatheca and are necessary for generating an organ with the normal number of cells. jun-1 is expressed in all cells of the developing spermatheca. We also provide evidence that NHR-6 and JUN-1 can physically interact in a yeast two-hybrid assay. Our results provide in vivo evidence that NR4A nuclear receptor and Jun transcription factor interactions are essential in regulating developmental processes in metazoans.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::45b0a755c19ac5fbd6af5adadd3c9126 https://pubmed.ncbi.nlm.nih.gov/24178943 Zobrazit plný text záznamu Plný text