| Autor: |
Sunitha, Shiva, Hieu Sy, Vu, Mary R, Roth, Zhenguo, Zhou, Shantan Reddy, Marepally, Daya Sagar, Nune, Gerald H, Lushington, Mahesh, Visvanathan, Ruth, Welti |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1009 |
| ISSN: |
1940-6029 |
| Popis: |
Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer's electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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