| Autor: |
Lisa S, Mydy, Robert W, Hoppe, Trevor M, Hagemann, Alan W, Schwabacher, Nicholas R, Silvaggi |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Biochemistry. 58(40) |
| ISSN: |
1520-4995 |
| Popis: |
The acetoacetate decarboxylase-like superfamily (ADCSF) is a little-explored group of enzymes that may contain new biocatalysts. The low level of sequence identity (∼20%) between many ADCSF enzymes and the confirmed acetoacetate decarboxylases led us to investigate the degree of diversity in the reaction and substrate specificity of ADCSF enzymes. We have previously reported on Sbi00515, which belongs to Family V of the ADCSF and functions as an aldolase-dehydratase. Here, we more thoroughly characterize the substrate specificity of Sbi00515 and find that aromatic, unsaturated aldehydes yield lower |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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