| Autor: |
Lotte, Hatt, Marie, Brinch, Ripudaman, Singh, Kristine, Møller, Rune Hoff, Lauridsen, Jacob Mørup, Schlütter, Niels, Uldbjerg, Britta, Christensen, Steen, Kølvraa |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Prenatal diagnosis. 34(11) |
| ISSN: |
1097-0223 |
| Popis: |
Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking.Blood samples from 78 normal pregnancies were used for testing the marker-set CD105 and CD141 for fetal cell enrichment. Fetal cell candidates were subsequently stained by a cocktail of cytokeratin antibodies, and the gender of the fetal cells was explored by fluorescence in situ hybridization (FISH) of the X and Y chromosomes.Fetal cell candidates could be detected in 91% of the samples, and in 85% of the samples, it was possible to obtain X and Y chromosomal FISH results for gender determination. The concordance between gender determined by FISH on fetal cells in maternal blood and gender found at birth reached 100% if three or more fetal cells with FISH signals could be found in a sample.The marker set identifies fetal cells with specificity high enough to make cell-based noninvasive prenatal diagnosis realistic. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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