[Isolation and characteristics of new restriction endonucleases from Haemophilus influenzae]

Autor:	L G, Lazariavichiute, Z P, Manialene, A M, Padegimene, L Iu, Kiudulene, V S, Lauchis, Iu B, Bitinaĭte, I M, Gruber, V M, Poliachenko, V V, Butkus, A A, Ianulaĭtis
Rok vydání:	1990
Předmět:	DNA Bacterial Base Sequence Hydrolysis Molecular Sequence Data Electrophoresis Gel Two-Dimensional DNA Restriction Enzymes Haemophilus influenzae Chromatography DEAE-Cellulose Plasmids Substrate Specificity
Zdroj:	Bioorganicheskaia khimiia. 16(7)
ISSN:	0132-3423
Popis:	Various strains of Haemophilus influenzae have been examined for the presence of site-specific endonuclease activities, and eleven restriction endonucleases have been isolated from seven strains. For all the endonucleases recognition sequences were determined, for three of them cleavage sites being identified. The enzymes proved to be isoschizomers of known endonucleases, viz. Hin1 I, Hin8 I--Acy I; Hin1 II, Hin8 II--Nla III; Hin2 I, Hin5 I--Hpa II; Hin3 I--Cau II; Hin5 II--Asu I; Hin5 III--Hind III; Hin6 I, Hin7 I--Hha I. Restriction endonucleases Hin1 I, Hin1 II and Hin6 I recognize nucleotide [formula: see text] sequences 5'GRCGPYC, 5'CATG, 5'GCGC, respectively, and cleave them as indicated by arrows.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::44e55de4eda58db81b1324e04c4f98cf https://pubmed.ncbi.nlm.nih.gov/2173603 Zobrazit plný text záznamu