Elements in the first intron of the alpha 1(I) collagen gene interact with Sp1 to regulate gene expression
| Autor: | D J, Liska, V R, Robinson, P, Bornstein |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Binding Sites
Base Sequence Sp1 Transcription Factor Molecular Sequence Data DNA Fibroblasts Regulatory Sequences Nucleic Acid Research Papers Introns Cell Line Gene Expression Regulation Mutagenesis Site-Directed Animals Humans Collagen Promoter Regions Genetic Chickens Sequence Alignment Protein Binding |
| Zdroj: | Gene expression. 2(4) |
| ISSN: | 1052-2166 |
| Popis: | Sequences within the first intron of the alpha 1(I) collagen gene act both positively and negatively to regulate expression of the gene. We have further characterized a 274 bp intronic sequence that contains an orientation-specific inhibitory activity and represents a constitutive DNase I-hypersensitive site in the gene. We show that this sequence contains two tandem, unique binding elements for the transcription factor Sp1. In addition, an Sp1-like site, capable of competing for protein binding to the intronic elements, resides in the distal promoter of the collagen gene. The results of experiments with site-directed mutations that abolish binding to the intronic elements indicate that these protein-DNA interactions have an inhibitory effect on the transcriptional efficiency of alpha 1(I) collagen-reporter gene constructs in transient transfection analysis. These data support our conclusion that the first intron plays a complex role, involving multiple protein-DNA binding interactions, in the regulation of expression of the alpha 1(I) collagen gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|