| Autor: |
M, Matos Neto, S S F, Santos, M V P, Leão, S M, Habitante, J R D D, Rodrigues, A O C, Jorge |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
International endodontic journal. 45(5) |
| ISSN: |
1365-2591 |
| Popis: |
To assess the effectiveness of three systems of mechanical preparation to reduce Enterococcus faecalis within root canals.Twenty-four human single-rooted canine teeth were standardized to a length of 17 mm and the canal contents removed using a size 20 K-file, as the last apical file. After irrigation and sterilization, the canals were contaminated with E. faecalis and incubated for 21 days at 37 °C with 5% CO(2). Then, the teeth were divided into three groups for mechanical preparation with: ProTaper rotary system, ProTaper manual system and manual K-files. Samples of the root canal contents, before and after the debridement, were collected with sterile paper points for 1 min. Then, the samples were diluted and plated in Brain Heart Infusion (BHI) agar. The colony-forming units were counted and the percentage reduction calculated. The reduction and log CFU mL(-1) were compared between groups using Wilcoxon nonparametric test and two-way analysis of variance, respectively.There was a significant reduction in the number of CFU/mL (P = 0.000) before and after debridement for all the systems used. However, there was no significant difference between the systems.All the three instrumentation systems reduced E. faecalis counts to a similar degree. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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