| Autor: |
Z H, Wang, M X, Ding, S B, Chew-Cheng, J P, Yun, E C, Chew |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 19(6B) |
| ISSN: |
0250-7005 |
| Popis: |
Bcl-2 and Bax proteins are implicated in the regulation of apoptosis. Nuclear matrix has been demonstrated to be associated with a vast array of functional and regulatory properties of cells. NuMA is one member of a class of nuclear matrix proteins that resides in both the nucleus and mitotic apparatus. The nuclear lamins appear to form a thin fibrous structure immediately underlying the inner nuclear membrane of eukaryotic cell nuclei. The association of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U343 was studied by confocal microscopy and Western blotting. Confocal microscopic images display that bcl-2 was localized at the peripheral of the nuclear matrix and Bax protein was located in the nuclear matrix. Western blotting detected a 26 kDa bcl-2 band and a specific band of Bax at around 66 kDa in nuclear matrix proteins. Our results suggest that bcl-2 and Bax proteins are nuclear matrix associated proteins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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