| Autor: |
Mona, El-Bahrawy, Richard, Poulsom, Andrew J, Rowan, Ian T M, Tomlinson, Malcolm R, Alison, S Richard, Poulsom, Ian T, Tomlinson |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
International journal of experimental pathology. 85(2) |
| ISSN: |
0959-9673 |
| Popis: |
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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