Sequences of Saccharomyces cerevisiae 2 microns DNA improving plasmid partitioning in Hansenula polymorpha

Autor:	A I, Bogdanova, O S, Kustikova, M O, Agaphonov, M D, Ter-Avanesyan
Rok vydání:	1998
Předmět:	Base Sequence Restriction Mapping Mitosis Deoxyribonuclease HindIII Saccharomyces cerevisiae Sequence Analysis DNA Pichia Meiosis Transformation Genetic DNA Fungal Deoxyribonucleases Type II Site-Specific Plasmids Repetitive Sequences Nucleic Acid Sequence Deletion
Zdroj:	Yeast (Chichester, England). 14(1)
ISSN:	0749-503X
Popis:	Insertion of the HindIII-PstI fragment of Saccharomyces cerevisiae 2 microns DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is caused by the improvement of plasmid partitioning. Deletion analysis revealed that the region of 2 microns DNA sequence responsible for the increase of mitotic stability of H. polymorpha plasmids involves the 2 microns STB locus and adjoining region. Further analysis demonstrated that the stabilization effect may depend on the number of 24-28 bp imperfect repeats which were found in several copies in the STB locus and adjoining region.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::44394f1253e7ba66a7af33d6260a5ec7 https://pubmed.ncbi.nlm.nih.gov/9483791 Zobrazit plný text záznamu Plný text