The protein kinase CK2 substrate Jabba modulates lipid metabolism during
| Autor: | Emily A, McMillan, Sheila M, Longo, Michael D, Smith, Sarah, Broskin, Baicheng, Lin, Nisha K, Singh, Todd I, Strochlic |
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| Rok vydání: | 2017 |
| Předmět: |
Recombinant Fusion Proteins
Green Fluorescent Proteins Ovary Gene Expression Regulation Developmental Lipid Metabolism Peptide Fragments Substrate Specificity Animals Genetically Modified Mice Drosophila melanogaster HEK293 Cells Oogenesis Microscopy Fluorescence 3T3-L1 Cells Animals Drosophila Proteins Humans Female RNA Interference Carrier Proteins Casein Kinase II Crosses Genetic Signal Transduction |
| Zdroj: | The Journal of biological chemistry. 293(8) |
| ISSN: | 1083-351X |
| Popis: | Lipid metabolism plays a critical role in female reproduction. During oogenesis, maturing oocytes accumulate high levels of neutral lipids that are essential for both energy production and the synthesis of other lipid molecules. Metabolic pathways within the ovary are partially regulated by protein kinases that link metabolic status to oocyte development. Although the functions of several kinases in this process are well established, the roles that many other kinases play in coordinating metabolic state with female germ cell development are unknown. Here, we demonstrate that the catalytic activity of casein kinase 2 (CK2) is essential for Drosophila oogenesis. Using an unbiased biochemical screen that leveraged an unusual catalytic property of the kinase, we identified a novel CK2 substrate in the Drosophila ovary, the lipid droplet–associated protein Jabba. We show that Jabba is essential for modulating ovarian lipid metabolism and for regulating female fertility in the fly. Our findings shed light on a CK2-dependent signaling pathway governing lipid metabolism in the ovary and provide insight into the long-recognized but poorly understood association between energy metabolism and female reproduction. |
| Databáze: | OpenAIRE |
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