Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes
| Autor: | S P D, Ponamgi, S, Rahamathulla, Y N, Kumar, M, Chandra, N, Lakshmi, C M, Habibullah, M N, Khaja |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Genotype Reverse Transcriptase Polymerase Chain Reaction Age Factors India Enzyme-Linked Immunosorbent Assay HIV Infections Comorbidity Hepacivirus Sequence Analysis DNA Hepatitis C Antibodies Middle Aged Viral Nonstructural Proteins Hepatitis C Young Adult Prevalence Humans RNA Viral Female Polymorphism Restriction Fragment Length |
| Zdroj: | Indian journal of medical microbiology. 27(1) |
| ISSN: | 1998-3646 |
| Popis: | To determine anti-HCV antibodies and genomic subtype of HCV in 1487 confirmed human immunodeficiency virus (HIV) positive samples.A total of 1487 confirmed HIV-positive samples were tested for anti-HCV antibodies by using a third generation ELISA kit (Ortho 3.0) and by RT PCR for HCV. HIV and HCV coinfected samples were selected for HCV genotyping by RFLP and subtyping with NS5-type specific primers.A total of 1487 HIV-infected serum samples were screened for HCV infection, of which, a 1443 (97.04%) were negative and 45 (3.02%) were coinfected. HIV-HCV coinfection was predominant in the age group 41-50 years (51.1%). HCV genotyping and subtyping was done for the 45 HCV RNA-positive specimens of which genotype 1 was observed in 31 (68.8%) and genotype 3 was observed in 14 (31.1%) subjects. Further subtyping analysis showed the genotype 1b in 23 (51.1%), 1a in eight (17.7%), 3a in 10 (22.2%) and 3b in four (8.8%) subjects.HIV and HCV seroprevalence is higher in South India, and the most prevalent genotype in coinfection was genotype 1b. |
| Databáze: | OpenAIRE |
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