| Autor: |
Saeeda, Bano, Samina, Iqbal, Kauser, Siddiqui, Kanwal, Abbasi |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Pakistan journal of pharmaceutical sciences. 34(4) |
| ISSN: |
1011-601X |
| Popis: |
Extra cellular β-galactosidase enzyme was purified and characterized from Aspergillus fumigatus PCSIR- 2013. Estimated molecular mass of the enzyme was approximately 95 kDa. by native polyacrylamide gel electrophoresis. Initially, different fermentation parameters were optimized for maximum production of β-galactosidase. The kinetic study of the partially purified enzyme exhibited that it remained active in broad range of temperature from 25°C to 70°C with an optimum of 60°C. The Km and Vmax were calculated as 9.95mmol/l and 51.78 U/ml/min, respectively. The optimum pH was 5.0, when reaction mixture was incubated for 30 min. The enzyme was very stable in the presence of different metal ions, although Na |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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