Replication asynchrony increases in women at risk for aneuploid offspring

Autor:	A, Amiel, O, Reish, E, Gaber, I, Kedar, R, Diukman, M, Fejgin
Rok vydání:	2000
Předmět:	Adult DNA Replication Male Chromosomes Human Pair 21 Centromere Mothers Middle Aged Aneuploidy Fathers Nondisjunction Genetic Risk Factors Humans Female Lymphocytes Down Syndrome Chromosomes Human Pair 18 Alleles In Situ Hybridization Fluorescence Chromosomes Human Pair 8 Microsatellite Repeats
Zdroj:	Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology. 8(2)
ISSN:	0967-3849
Popis:	We attempted to demonstrate a relation between a loss of replication control, centromere dysfunction, and predisposition to non-disjunction. Couples with a Down syndrome offspring were the high-risk probands. One-color FISH (fluorescent in-situ hybridization) was applied to interphase nuclei (lymphocytes). Replication pattern of two pairs of alleles, RB-1 and 21q22, were studied, and the rate of aneuploidy was estimated using two alpha-satellite probes of chromosomes 8 and 18. Our results suggest the existence of an association between replication timing and the rate of non-disjunction. A higher rate of allele asynchrony and aneuploidy was found in older women and in mothers of a Down syndrome offspring. These findings may reflect a predisposition for meiotic non-disjunction in these women.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::43d2c8ff79254994485a768e649b4300 https://pubmed.ncbi.nlm.nih.gov/10780703 Zobrazit plný text záznamu Full text from SpringerLink