| Autor: |
Lei, Chen, Xiao-Sheng, Lu, Ya-Lan, Li, Zhou-Fei, Mao, Luan-Juan, Xiao, Yan-Hong, Yu |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 37(5) |
| ISSN: |
1673-4254 |
| Popis: |
OBJECTIVE: To analyze the effect of globular adiponectin on angiogenesis of ovarian microvascular endothelial cells (OMECs). METHODS: Mouse OMECs were isolated and purified by density gradient centrifugation with Percoll and identified by immunofluorescence analysis of follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and endothelial cell marker von Willebrand factor (vWF). The capillary-like tube formation of OMECs was determined by vascular endothelial growth factor A (VEGFA) treatment in Matrigel matrix. OMECs treated with recombinant globular adiponectin protein were examined for cell proliferation with MTS assay and cell migration with scratch wound healing assay, and capillary-like tube formation was tested in Matrigel matrix. Western blotting was performed to detect the effect of globular adiponectin on AMPK phosphorylation. RESULTS: The signals of LHR and vWF, but not that of FSHR, were detected in the isolated cells. VEGFA treatment of the cells induced capillary-like tube formation, indicating their properties of ovarian-specific endothelial cells. Treatment with 1 and 3 μg/mL of recombinant globular adiponectin significantly increased the number of OMECs by (158.72±14.50) % and (186.50±4.20) % (P < 0.01) and resulted in scratch wound closure rates of (49.43±3.43) % (P < 0.05) and (69.67±1.2) % (P < 0.01) respectively. The cells treated with 3 μg/mL globular adiponectin formed a capillary-tube length 6.63±0.66 folds greater than that formed by the control cells (P < 0.01). Treatment of the cells with 3 μg/mL globular adiponectin for 15 and 30 min resulted in pAMPK/ AMPK ratios of 0.86 ± 0.08 and 0.66 ± 0.13, respectively significantly higher than that in the control cells (0.13 ± 0.12, P < 0.01). Compound C obviously suppressed the tube formation and AMPK phosphorylation induced by globular adiponectin. CONCLUSION: Globular adiponectin promotes angiogenesis of OMECs through activation of the AMPK signal pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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