Secretion of cytokines by human synoviocytes during in vitro infection with Chlamydia trachomatis
| Autor: | J, Rödel, E, Straube, W, Lungershausen, M, Hartmann, A, Groh |
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| Rok vydání: | 1998 |
| Předmět: |
Bacteria
Interleukin-6 Tumor Necrosis Factor-alpha Synovial Membrane Granulocyte-Macrophage Colony-Stimulating Factor Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay Chlamydia Infections Fibroblasts Interferon-gamma Transforming Growth Factor beta Cytokines Humans Biological Assay Cells Cultured |
| Zdroj: | The Journal of rheumatology. 25(11) |
| ISSN: | 0315-162X |
| Popis: | Since Chlamydia-induced reactive arthritis is associated with the presence of viable chlamydiae in the synovial membrane, we studied the ability of Chlamydia trachomatis to stimulate a cytokine response by fibroblast-like synoviocytes in culture.Fibroblast-like cells derived from biopsies of the synovial membrane were infected with Chlamydia trachomatis serotype E. Interleukin-6 (IL-6), transforming growth factor-beta (TGF-beta) and tumor necrosis factor-alpha (TNF-alpha) were determined using bio-assays. Granulocyte macrophage colony stimulating factor (GMCSF) was quantified by ELISA.Fibroblast-like synovial cells were capable of supporting chlamydial growth in vitro. Chlamydia trachomatis stimulated synoviocytes to produce IL-6, TGF-beta, and GMCSF. IL-1beta increased the production of IL-6 and GMCSF by mock-infected and infected cells. Treatment of synoviocytes with interferon-gamma resulted in the release of TNF-alpha in response to chlamydial infection.Chlamydia-induced cytokine release from synovial fibroblasts may contribute to alterations in the synovial membrane promoting the development of joint inflammation. |
| Databáze: | OpenAIRE |
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