Cloning and characterization of 5'-end alternatively spliced human cholecystokinin-B receptor mRNAs
| Autor: | H J, Monstein, I, Nilsson, K, Ellnebo-Svedlund, S P, Svensson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Benzodiazepinones
Base Sequence Phenylurea Compounds Molecular Sequence Data Sequence Analysis DNA Transfection Binding Competitive Alternative Splicing Protein Biosynthesis COS Cells Gastrins Animals Humans Receptors Cholecystokinin Amino Acid Sequence RNA Messenger Cloning Molecular Cholecystokinin Protein Binding |
| Zdroj: | Receptorschannels. 6(3) |
| ISSN: | 1060-6823 |
| Popis: | We report here the cloning and characterization of a 5'-end alternatively spliced human cholecystokinin-B (CCK-B) receptor mRNA. The 5'-end of this CCK-B receptor transcript (termed CCK-BRtx) consisted of exon Ia, present in the ordinary full-length CCK-B receptor mRNA (CCK-BRwt), and exon Ib, present in a previously described 5'-end alternatively spliced CCK-B receptor mRNA (CCK-BRt). A short open reading frame preceded the AUG translation initiation codon of the CCK-BRtx. Transfection of COS-7 cells with the CCK-BRtx or CCK-BRt cDNAs did not lead to the appearance of peptidergic and non-peptidergic binding sites. Cell free in vitro translation yielded proteins of approximately 44 kDa (CCK-B receptor) and 40 kDa (CCK-BRt receptor) whereas no 40 kDa product was detected from the cloned CCK-BRtx cDNA. Instead, a protein product of approximately 9 kDa was visualized, the size corresponding to the predicted protein encoded by the short open reading frame. The alternatively spliced CCK-B receptor transcripts were concomitantly expressed with the ordinary full-length CCK-B receptor mRNA in the brain, pancreas, and stomach. The possibility that such transcripts are translated in vivo into truncated CCK-B receptors is discussed. |
| Databáze: | OpenAIRE |
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