Molecular genetics analysis for co-infection of Brugia malayi and Brugia pahangi in cat reservoirs based on internal transcribed spacer region 1

Autor:	Supatra, Areekit, Sintawee, Khuchareontaworn, Pornpimon, Kanjanavas, Thayat, Sriyapai, Arda, Pakpitchareon, Paisarn, Khawsak, Kosum, Chansiri
Rok vydání:	2009
Předmět:	Base Sequence Sequence Analysis DNA DNA Helminth Cat Diseases Polymerase Chain Reaction Brugia pahangi Filariasis DNA Ribosomal Spacer Cats Animals Brugia malayi Phylogeny Polymorphism Restriction Fragment Length Disease Reservoirs
Zdroj:	The Southeast Asian journal of tropical medicine and public health. 40(1)
ISSN:	0125-1562
Popis:	This study described the diagnosis of a mixed infection of Brugia malayi and Brugia pahangi in a single domestic cat using the internal transcribed spacer 1 (ITS1) region. Following polymerase chain reaction amplification of the ITS1 region, the 580 bp amplicon was cloned, and 29 white colonies were randomly selected for DNA sequencing and phylogenetic tree construction. A DNA parsimony tree generated two groups of Brugia spp with one group containing 6 clones corresponding to B. pahangi and the other 23 clones corresponding to B. malayi. This indicated that mixed infection of the two Brugia spp, B. pahangi and B. malayi, had occurred in a single host.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::431efac7369b875eace23eafc5813982 https://pubmed.ncbi.nlm.nih.gov/19323030 Zobrazit plný text záznamu