[Mycoplasma restriction-modification system MunI and its possible role in pathogenesis processes]
| Autor: | P S, Stakenas, N M, Zaretskaia, Z P, Manelene, M M, Mauritsas, V V, Butkus, A A, Ianulaĭtis |
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| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Electrophoresis Agar Gel Acquired Immunodeficiency Syndrome Base Sequence Molecular Sequence Data Bacteriophage lambda Mice DNA Viral Tumor Cells Cultured Animals Chromatography Thin Layer Leukemia Erythroblastic Acute Mycoplasma fermentans Deoxyribonucleases Type II Site-Specific Chromatography High Pressure Liquid |
| Zdroj: | Molekuliarnaia biologiia. 26(3) |
| ISSN: | 0026-8984 |
| Popis: | The restriction-modification system, named RMMunI, has been purified and characterised from Friend murine erythroleukemia cells. The site-specific endonuclease recognizes and cleaves the 5'C1AATTG nucleotide sequence. RMunI is an isoschizomer of RMfeI from Mycoplasma fermentans. Site-specific methylase modifies the second adenine residue in the same sequence (5'Cam6ATTG). It was established that the discovered enzymatic system is from mycoplasma which contaminates cell lines. Mycoplasma's DNA hybridizes with species-specific DNA probed for Mycoplasma fermentans and Mycoplasma arginini. The possible role of mycoplasmic restriction-modification enzymes in the process of acquired immune deficiency syndrome are discussed. |
| Databáze: | OpenAIRE |
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