[The isolation of monoclonal antibodies to hepatoma-associated nonhistone chromosomal proteins from rat kidneys]

Autor:	A B, Grandilevskaia, D G, Polyntsev, V P, Kushner, V A, Ivanov
Rok vydání:	1993
Předmět:	Male Mice Inbred BALB C Hybridomas Chromosomal Proteins Non-Histone Antibodies Monoclonal Fluorescent Antibody Technique Kidney Rats Molecular Weight Mice Liver Neoplasms Experimental Liver Antigens Neoplasm Antigens Heterophile Animals Immunization
Zdroj:	Tsitologiia. 35(9)
ISSN:	0041-3771
Popis:	A monoclonal antibody (5D2) has been produced to a fraction of nonhistone proteins of kidney origin, associated with the rat hepatoma 27 and not obtained in normal liver. The 5D2 antibody was found to precipitate three proteins with molecular weights of 22-23 kDa. Indirect immunofluorescence studies on fixed hepatoma cell-preparations showed speckled nuclear staining patterns. In contrast, the 5D2 antibody was unreactive with intact hepatocytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4317862a6031cf781f84f4cbfed73ab2 https://pubmed.ncbi.nlm.nih.gov/8266582 Zobrazit plný text záznamu