| Autor: |
Qing, Luo, Jia-Ling, Zhong, Mei, Zhao, Yi-Ming, Ma, Jian, Liu, Jia, Wang, Chang-Zhi, Huang |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 27(2) |
| ISSN: |
1007-8738 |
| Popis: |
To investigate the effects of NAIF1 on biological behavior of esophageal carcinoma cell line EC9706 by using pTet-off system.Constructe the pTRE2-NAIF1 plasmid and co-transfected with PTK-Hyg in human inducable esophageal carcinoma cell line TF93 (derive from EC9706 cell line).After Hygromycin B selection and RT-PCR, Western blot identification we got stable inducable cell clone TF93-pTRE2-NAIF1.By using this clone, we used MTT to test the proliferation rate, flow cytometry to test the cell cycle, Transwell chamber to test the migration ability and tested the adhesion ability of EC9706 under the effect of NAIF1.First we got the stable inducable cell clone TF93-pTRE2-NAIF1, then we found NAIF1 could inhibit the cell proliferation, cause G0/G1 cell arrest and inhibit the migration and adhesion ability of EC9706.NAIF1 can inhibit the malignant phenotype of esophageal carcinoma cell line EC9706. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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