| Autor: |
M, Esclapez, D K, Chang, C R, Houser |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Hippocampus. 6(3) |
| ISSN: |
1050-9631 |
| Popis: |
The alpha 1 subunit of the gamma-aminobutyric acid (GABA)A receptor is highly expressed in a subgroup of neurons in the hippocampal formation. The distribution and chemical identities of these neurons in the dentate gyrus have been studied with double-labeling in situ hybridization and immunohistochemical methods. Double labeling for the alpha 1 subunit and glutamate decarboxylase 65 (GAD65) mRNAs indicated that virtually all neurons in the dentate gyrus that are heavily labeled for the alpha 1 subunit are GABA neurons. However, many GAD65 mRNA-labeled neurons in the hilus do not contain high levels of the alpha 1 subunit mRNA and protein. Studies were thus conducted to determine if the somatostatin neurons of the hilus were part of the alpha 1 subunit-labeled group. Double labeling for the alpha 1 subunit and pre-prosomatostatin mRNAs demonstrated virtually no co-localization of these mRNAs in hilar neurons. Thus, the strongly labeled alpha 1 mRNA-containing neurons and the somatostatin neurons constitute two distinct populations of hilar GABA neurons. Double labeling for the alpha 1 subunit polypeptide and its mRNA with immunohistochemical and in situ hybridization methods demonstrated directly that neurons of the dentate gyrus that express high levels of the alpha 1 subunit mRNA are the same neurons that show extensive labeling for the alpha 1 subunit along their somal and dendritic surfaces. The high levels of alpha 1 subunit expression in some populations of GABA neurons could be related to prominent disinhibitory functions of these neurons. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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