454-Sequencing™ for the KEL, JR, and LAN Blood Groups

Autor:	Carola, Wieckhusen, Peter, Bugert
Rok vydání:	2015
Předmět:	Genotyping Techniques Kell Blood-Group System Blood Group Antigens High-Throughput Nucleotide Sequencing Humans Sequence Analysis DNA Validation Studies as Topic Nucleic Acid Denaturation Polymerase Chain Reaction
Zdroj:	Methods in molecular biology (Clifton, N.J.). 1310
ISSN:	1940-6029
Popis:	The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-sequencing method using 454-sequencing™ on a Genome Sequencer (GS) Junior™ system. Multiplex analysis of eight individual DNA samples was achieved using molecular identifiers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::428288ca91750c0993bf842406f3eed3 https://pubmed.ncbi.nlm.nih.gov/26024631 Zobrazit plný text záznamu