| Autor: |
N Yu, Vorobyeva, N S, Babayan, B A, Grigoryan, A A, Sargsyan, L G, Khondkaryan, L S, Apresyan, A K, Chigasova, E I, Yashkina, D V, Guryev, S M, Rodneva, A A, Tsishnatti, Yu A, Fedotov, R M, Arutyunyan, A N, Osipov |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Bulletin of experimental biology and medicine. 172(6) |
| ISSN: |
1573-8221 |
| Popis: |
We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink