| Autor: |
Jacob W, McCabe, Benjamin J, Jones, Thomas E, Walker, Robert L, Schrader, Adam P, Huntley, Jixing, Lyu, Nathan M, Hoffman, Gordon A, Anderson, Peter T A, Reilly, Arthur, Laganowsky, Vicki H, Wysocki, David H, Russell |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
J Am Soc Mass Spectrom |
| ISSN: |
1879-1123 |
| Popis: |
Here, we describe a digital-waveform dual-quadrupole mass spectrometer that enhances the performance of our drift tube FT-IMS high-resolution Orbitrap mass spectrometer (MS). The dual-quadrupole analyzer enhances the instrument capabilities for studies of large protein and protein complexes. The first quadrupole (q) provides a means for performing low-energy collisional activation of ions to reduce or eliminate noncovalent adducts, viz., salts, buffers, detergents, and/or endogenous ligands. The second quadrupole (Q) is used to mass-select ions of interest for further interrogation by ion mobility spectrometry and/or collision-induced dissociation (CID). Q is operated using digital-waveform technology (DWT) to improve the mass selection compared to that achieved using traditional sinusoidal waveforms at floated DC potentials (>500 V DC). DWT allows for increased precision of the waveform for a fraction of the cost of conventional RF drivers and with readily programmable operation and precision (Hoffman, N. M. et al.. A comparison-based digital-waveform generator for high-resolution duty cycle. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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