The centromere-binding factor Cbf1p from Candida albicans complements the methionine auxotrophic phenotype of Saccharomyces cerevisiae
| Autor: | R, Eck, T, Stoyan, W, Künkel |
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| Rok vydání: | 2001 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Genes Fungal Molecular Sequence Data Mitosis Saccharomyces cerevisiae Fungal Proteins Methionine Transformation Genetic Sequence Homology Nucleic Acid Candida albicans Amino Acid Sequence Isoelectric Point Cloning Molecular DNA Fungal Promoter Regions Genetic Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Genetic Complementation Test Chromosome Mapping Sequence Analysis DNA DNA-Binding Proteins Molecular Weight Phenotype Mutation Chromosomes Fungal Sequence Alignment Plasmids |
| Zdroj: | Yeast (Chichester, England). 18(11) |
| ISSN: | 0749-503X |
| Popis: | A gene encoding the centromere binding factor 1 (Cbf1p) of the human pathogenic yeast Candida albicans was cloned and characterized. An open reading-frame was detected which encoded a 223 amino acid protein with a calculated molecular weight of 25.8 kDa and a relative isoelectric point of 5.55. It shares 39% overall amino acid sequence identity with Saccharomyces cerevisiae Cbf1p. We localized the CaCBF1 gene on chromosome 4. Southern analysis indicated that CaCBF1 is probably present as a single copy gene per haploid genome. The CaCBF1 gene under the control of its own promoter was able to complement the methionine auxotrophic growth, the increased mitotic instability of CEN plasmids and the slow growth of a Saccharomyces cerevisiae cbf1Delta mutant strain. |
| Databáze: | OpenAIRE |
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