Volume regulation of spermatozoa by quinine-sensitive channels

Autor:	S B, Kulkarni, Z E, Sauna, V, Somlata, V, Sitaramam
Rok vydání:	1997
Předmět:	Male Oxygen Consumption Potassium Channels Buffaloes Quinine Osmolar Concentration Sperm Motility Animals Cattle Sperm Capacitation Spermatozoa Cells Cultured Culture Media
Zdroj:	Molecular reproduction and development. 46(4)
ISSN:	1040-452X
Popis:	Bovine spermatozoa were shown to exhibit rapid regulatory volume decrease (RVD) when exposed to hypotonic saline media. This quinine- and quinidine-sensitive regulatory volume decrease was coincident with K+ release due to stretch-activation of inhibitor-specific presumptive K+ channels. The regulatory volume decrease response was much faster than a similar phenomenon observed in human peripheral blood lymphocytes. Studies on volume changes in different electrolyte and nonelectrolyte media suggested that: (1) this inhibitor-specific channel could also be a nonspecific pore in the spermatozoal membrane for nonelectrolytes below 150 daltons; (2) subpopulations (of nearly equal size) of the spermatozoa differ in the expression of the pore; (3) capacitation abolishes this distinction between subpopulations of spermatozoa; and (4) the general case of RVD for other mammalian spermatozoa was also established.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::420b5ae7c8c8fe4881b12a7c6b5c94b9 https://pubmed.ncbi.nlm.nih.gov/9094101 Zobrazit plný text záznamu Plný text