[Transfer of bacterial genes for proline synthesis in plants and their expression by various plant promotors]
| Autor: | A, Sokhansandzh, L V, Neumyvakin, N A, Moseĭko, E S, Piruzian |
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| Rok vydání: | 1997 |
| Předmět: |
Proline
Genetic Vectors Gene Transfer Techniques Phosphotransferases (Carboxyl Group Acceptor) Plants Genetically Modified Aldehyde Oxidoreductases Plants Toxic Transformation Genetic Agrobacterium tumefaciens Gene Expression Regulation Plant Genes Bacterial Tobacco Escherichia coli Glutamate-5-Semialdehyde Dehydrogenase Promoter Regions Genetic Plasmids |
| Zdroj: | Genetika. 33(7) |
| ISSN: | 0016-6758 |
| Popis: | Accumulation of free proline (Pro) by bacteria and plants serves as a mechanism protecting these organisms from abiotic stress. We introduced two E. coli genes, proBosm, encoding a mutant variant of the first enzyme of the Pro biosynthetic pathway, gamma-glutamyl kinase, and proA, encoding the second enzyme of the Pro pathway, glutamyl-gamma-semialdehyde dehydrogenase, into tobacco plants. The spontaneous proBosm mutation was selected for Pro overproduction and mapped to the N-terminus of a polypeptide chain; it was found to result from one amino-acid substitution. Two E. coli genes were simultaneously introduced into tobacco plants, each under the control of a strong constitutive CaMV 35S promoter, which contained a duplicated sequence of enhancer, or the root-specific Pmas promoter sequence. Transgenic plants were characterized by Pro overproduction, increased resistance to L-azetidine-2-carboxylic acid (a toxic analog of Pro), and tolerance to salt stress. |
| Databáze: | OpenAIRE |
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