| Autor: |
Masayasu, Bando, Mikako, Oka, Kenji, Kawai, Hajime, Obazawa, Makoto, Takehana |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Molecular vision. 13 |
| ISSN: |
1090-0535 |
| Popis: |
In the rabbit lens, high levels of reduced nicotinamide adenine dinucleotide (NADH) can function as a near-ultraviolet light (near-UV) filter, an effect apparently achieved by specific nucleotide binding to lambda-crystallin. The present investigation asks whether lambda-crystallin enhances NADH photo-oxidation by superoxide radicals produced via a photosensitization reaction of near-UV with NADH.Lambda-crystallin was partially purified from rabbit lens soluble fraction by a two-step gel filtration and affinity column chromatography procedure. NADH solutions with or without partially purified lambda-crystallin were subjected to near-UV irradiation or exposed to superoxide generated enzymatically by the xanthine/xanthine oxidase system. NADH oxidation was determined by assaying the decrease of absorbance at 340 nm.When irradiated with near-UV, free NADH was oxidized very little in the absence of lambda-crystallin. In contrast, NADH photo-oxidation was rapidly initiated in the presence of partially purified lambda-crystallin. This lambda-crystallin-enhanced NADH photo-oxidation was totally inhibited by adding superoxide dismutase. We also found that lambda-crystallin largely increased NADH oxidation by a superoxide that is generated enzymatically. These results indicate that NADH bound to lambda-crystallin rapidly reacts with superoxides. The reactivity of bound NADH with superoxide was almost equivalent to that of ascorbic acid. However, lambda-crystallin-enhanced NADH oxidation exceeded the superoxide levels generated by NADH photosensitization and xanthine/xanthine oxidase.We conclude that NADH binding to lambda-crystallin enhances NADH photo-oxidation through a radical chain reaction mechanism that is initiated by superoxides generated by NADH photosensitization and propagated by another superoxide from the molecule oxygen. High concentrations of NADH bound to lambda-crystallin may be beneficial to the rabbit lens in scavenging the low amounts of superoxide produced by near-UV absorption, since oxygen tension in the lens is very low. We also discuss the function of near-UV-filtering and the anti-photo-oxidation systems in other vertebrate lenses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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