Whole genome sequencing of methicillin-resistant and methicillin-sensitive
| Autor: | Paloma, Morice, Marion, Allano, Chantale, Provost, Julie-Hélène, Fairbrother, Carl A, Gagnon, Frédéric, Sauvé |
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| Rok vydání: | 2020 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus Whole Genome Sequencing Virulence Factors Short Communication biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Anti-Bacterial Agents Hospitals Animal Methicillin Animals Horse Diseases Methicillin Resistance Horses Phylogeny |
| Zdroj: | Can J Vet Res |
| ISSN: | 1928-9022 |
| Popis: | Genomic characterization was conducted on 2 methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from 2 horses hospitalized during an overlapping period of time and 2 methicillin-sensitive S. aureus (MSSA) strains isolated from 2 distinct horses. Phylogenetic proximity was traced and the genotypic and phenotypic characteristics of the antimicrobial resistance of the strains were compared. Whole genome sequencing of MRSA strains for this report was similar but differed from whole genome sequencing of MSSA strains. The MRSA strains were closely related, belonging to sequence type (ST) 612, spa type t1257, and SCCmec type IVd2B. The MSSA strains were also closely related, belonging to ST1660, spa type t3043, and having no detectable staphylococcal cassette chromosome mec elements. All MSRA and MSSA strains were Panton-Valentine leukocidin negative. There were discrepancies in the genotypic analysis and the antimicrobial susceptibility testing (phenotypic analysis) of MRSA strains for rifampin, trimethoprim-sulfamethoxazole, gentamicin, amikacin, and enrofloxacin. |
| Databáze: | OpenAIRE |
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