Global assessment of cross-hybridization for oligonucleotide arrays

Autor:	Cavan, Reilly, Arvind, Raghavan, Paul, Bohjanen
Rok vydání:	2006
Předmět:	Proteomics Models Statistical CD3 Complex Computational Biology Nucleic Acid Hybridization Reproducibility of Results Genomics Articles CD28 Antigens Gene Expression Regulation Data Interpretation Statistical Humans Lymphocytes Oligonucleotide Probes Software Oligonucleotide Array Sequence Analysis
Zdroj:	Journal of biomolecular techniques : JBT. 17(2)
ISSN:	1524-0215
Popis:	Cross-hybridization is the tendency for chains of nucleic acids to bind to other chains of nucleic acids that have similar but not identical sequences. This has the potential to make the interpretation of microarray experiments difficult since intensity at a spot on the array does not simply depend on the quantity of target in the sample. We propose a method for evaluating the extent of cross-hybridization in oligonucleotide arrays for data arising from a typical microarray experiment. We show that the level of cross-hybridization can be quite substantial. We argue that this makes the interpretation of the difference calls provided by MAS 5.0 difficult.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::41a9edb442001fd7e2f854163e2111a7 https://pubmed.ncbi.nlm.nih.gov/16741244 Zobrazit plný text záznamu