Analgesic, Anti-Inflammatory, and Antioxidant Activities of

Autor: Maria Christina Dos Santos, Verdam, Fernanda, Guilhon-Simplicio, Kleyton Cardoso, de Andrade, Karina Lorena Meira, Fernandes, Tallita Marques, Machado, Felipe Moura Araújo, da Silva, Mayane Pereira, de Souza, Hector Henrique Ferreira, Koolen, Cristiane da Silva, Paula, Beatriz Cristina Konopatzki, Hirota, Vinícius Bednarczuk, de Oliveira, Cristina Mayumi Sasaki, Miyazaki, Milena, Kalegari, Marilis Dallarmi, Miguel, Patricia Maria, Stuelp-Campelo, Obdulio Gomes, Miguel
Rok vydání: 2016
Předmět:
Zdroj: The Scientific World Journal
ISSN: 1537-744X
Popis: Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by 57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples. FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg·kg−1) showed significant analgesic activity. Conclusion. B. duckeana has interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.
Databáze: OpenAIRE