| Autor: |
Xun, Jiang, Wenxian, Chen, Zhipei, Zhang, Dianzhong, Zhang, Pengcheng, Cui, Guize, Li, Pengfei, Gao, An, Sun |
| Rok vydání: |
2003 |
| Předmět: |
|
| Zdroj: |
Zhonghua er bi yan hou ke za zhi. 37(4) |
| ISSN: |
0412-3948 |
| Popis: |
To investigate the biologic characters of HPV positive laryngeal epithelial cells in vitro.To draw a cell growth curve by observing the biologic characters of HPV positive cells' growth pattern, PCR and nuclear acid hybridization were applied before and after culture for confirming HPV positive cells DNA.Primary HPV positive cells often remain viable in culture for more than six weeks, there have HPV DNA before and after culture. We found that in these cells had common characters: lag phase, growth phase and plateau phase. The cells culture showed that the first to forth day there have many cells emigration from tissue pieces. The fifth to Seventh day was latent phase, it was mainly adhere growth in this period, proliferation of culture cells wasn't active. The eighth to eighteenth day was growth phase, cells number was increased quickly, speed of growth was faster. Then cells growth entered to plateau phase, cells number was increased slowly, cells showed vacuoles obviously. It indicated that these cells came to die.Cells derives from HPV positive laryngeal epithelial have been successfully cultured, but to establish an animal model for HPV infection is difficult. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
