Chromosome Congression in the Absence of K-Fibres
| Autor: | Cai, Shang, O’Connell, Christopher B., Khodjakov, Alexey, Walczak, Claire E. |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Nature cell biology |
| ISSN: | 1476-4679 1465-7392 |
| Popis: | Proper chromosome congression (the process of aligning chromosomes on the spindle) contributes to accurate and faithful chromosome segregation. It is widely accepted that congression requires ‘K-fibres’, microtubule bundles that extend from the kinetochores to spindle poles1, 2. Here we demonstrate that chromosomes in human cells co-depleted for HSET (kinesin-14)3, 4 and hNuf2 (a component of the Ndc80/Hec1 complex)5 can congress to the metaphase plate in the absence of K-fibres. However, the chromosomes were not stably maintained at the metaphase plate under these conditions. Chromosome congression in HSET+hNuf2 co-depleted cells required the plus-end directed motor CENP-E (kinesin-7)6, which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres7. Thus, proper end-on attachment of kinetochores to microtubules is not necessary for chromosome congression. Instead, our data support the idea that congression allows unattached chromosomes to move to the middle of the spindle where they have a higher probability of establishing connections with both spindle poles. These bi-oriented connections are also utilized to maintain stable chromosome alignment at the spindle equator. |
| Databáze: | OpenAIRE |
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