Isolation of effector-selective Ras mutants by yeast two-hybrid screening

Autor:	K J, Kaur, M A, White
Rok vydání:	2001
Předmět:	Genes ras Phenotype Two-Hybrid System Techniques Mutation Saccharomyces cerevisiae Plasmids
Zdroj:	Methods in enzymology. 332
ISSN:	0076-6879
Popis:	Ras mutants displaying selective target interactions will often display partial loss of function phenotypes when expressed in cells. Thus the isolation of mutations in Ras and Ras family members has proved to be a productive approach for testing the requirement of specific target interactions to mediate downstream responses. The procedures outlined here greatly simplify the isolation of such mutants, and it is hoped will contribute to a better understanding of Ras effector function.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::40ee93d40fb58af6c6fd3501d337f0b9 https://pubmed.ncbi.nlm.nih.gov/11305103 Zobrazit plný text záznamu