The use of differential scanning calorimetry (DSC) to determine the correctness of folding of cloned proteins

Autor:	J M, Brewer
Rok vydání:	1999
Předmět:	Protein Denaturation Protein Folding Hot Temperature Calorimetry Differential Scanning Phosphopyruvate Hydratase Mutation Temperature Titrimetry Histidine Recombinant Proteins
Zdroj:	Biotechnology and applied biochemistry. 30(2)
ISSN:	0885-4513
Popis:	The advantages and limitations of use of the differential scanning calorimetry technique to determine whether cloned and expressed mutant proteins are folded correctly are explained and discussed. Examination of stabilization of these proteins to thermal denaturation by specific ligand binding can provide the required information.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4052ebc6c718bdbfd3aad5ac72242717 https://pubmed.ncbi.nlm.nih.gov/10512797 Zobrazit plný text záznamu Plný text